Article
Mutational mapping of RAS-responsive domains of the Saccharomyces cerevisiae adenylyl cyclase.
Molecular and cellular biology - 1 Jun 1990
Colicelli J, Field J, Ballester R, Chester N, Young D, Wigler M
Abstract excerpt
Large deletion and small insertion mutations in the adenylyl cyclase gene of Saccharomyces cerevisiae were used to map regions required for activation by RAS protein in vitro. The amino-terminal 605 amino acids were found to be dispensable for responsiveness to RAS protein. All other deletions in adenylyl cyclase destroyed its ability to respond to RAS. Small insertion mutations within the leucine-rich repeat...
Topics
- Adenylyl Cyclases
- Cloning, Molecular
- DNA, Fungal
- Escherichia coli
- Fungal Proteins
- GTP-Binding Proteins
- Genes, Fungal
- Molecular Weight
- Mutation
- Plasmids
- Restriction Mapping