Yeast model system for study of mammalian phosphodiesterases.

doi:10.1006/meth.1997.0563

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Article

Yeast model system for study of mammalian phosphodiesterases.

Methods (San Diego, Calif.) - 1 Jan 1998

Atienza J M, Colicelli J

Abstract excerpt

Facile manipulation and rapid regeneration have helped to establish the yeast Saccharomyces cerevisiae as a genetic workhorse. More recently, these simple eukaryotes have been used for the biochemical analysis of mammalian proteins. This article describes the use of a yeast expression system for...

Topics

Animals
Drug Resistance
Enzyme Inhibitors
Gene Expression
Genetic Vectors
Hot Temperature
Mammals
Phenotype
Phosphodiesterase Inhibitors
Phosphoric Diester Hydrolases
Saccharomyces cerevisiae
Transformation, Genetic

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