Yeast model system for study of mammalian phosphodiesterases.

Abstract excerpt

Facile manipulation and rapid regeneration have helped to establish the yeast Saccharomyces cerevisiae as a genetic workhorse. More recently, these simple eukaryotes have been used for the biochemical analysis of mammalian proteins. This article describes the use of a yeast expression system for both in vitro and in vivo assays of mammalian phosphodiesterase (PDE) activity using yeast cells devoid of endogenous...

Topics

Share this publication in a Topic to start or enrich a Post.

• Share to Drug Resistance
• Share to Enzyme Inhibitors
• Share to Gene Expression