Article
In vivo mutagenesis by Escherichia coli DNA polymerase I. Ile(709) in motif A functions in base selection.
The Journal of biological chemistry - 14 Dec 2001
Shinkai A, Loeb L A
Abstract excerpt
The fidelity of DNA replication by Escherichia coli DNA polymerase I (pol I) was assessed in vivo using a reporter plasmid bearing a ColE1-type origin and an ochre codon in the beta-lactamase gene. We screened 53 single mutants within the region Val(700)-Arg(712) in the polymerase active-site motif A. Only replacement of Ile(709) yielded mutator polymerases, with substitution of Met, Asn, Phe, or Ala increasing...
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