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[A temperature-inducible Targetron system for efficient gene inactivation in Escherichia coli].

Sheng wu gong cheng xue bao = Chinese journal of biotechnology - 25 Aug 2020

Zhao Xingxing, Cheng Yumei, Wu Changxue, Ren Wei, Rao Fengqin, Zhou Qian, Cui Guzhen, Qi Xiaolan, Hong Wei

Abstract excerpt

To construct TeI3c/4c-based and temperature-inducible gene inactivation system (Thermotargetron) and to apply it to gene inactivation of mesophilic bacteria. The subunit of flagellum (fliC) and C4 dicarboxylate orotate:H⁺ symporter (dctA) genes were chosen as targets in the genome of Escherichia coli HMS174 (DE3) strain. According to recognition roles of TeI3c/4c intron, the fliC489a, fliC828s, fliC1038s and...

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Identifiers

PMID: 32924364
DOI: 10.13345/j.cjb.190547

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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