Article
A thiostrepton resistance gene and its mutants serve as selectable markers in Geobacillus kaustophilus HTA426.
Bioscience, biotechnology, and biochemistry - 1 Jan 2016
Wada Keisuke, Kobayashi Jyumpei, Furukawa Megumi, Doi Katsumi, Ohshiro Takashi, Suzuki Hirokazu
Abstract excerpt
Effective utilization of microbes often requires complex genetic modification using multiple antibiotic resistance markers. Because a few markers have been used in Geobacillus spp., the present study was designed to identify a new marker for these thermophiles. We explored antibiotic resistance genes functional in Geobacillus kaustophilus HTA426 and identified a thiostrepton resistance gene (tsr) effective at...
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