Article
Distinct function of conserved amino acids in the fingers of Saccharomyces cerevisiae DNA polymerase alpha.
The Journal of biological chemistry - 23 May 2003
Ogawa Masanori, Limsirichaikul Siripan, Niimi Atsuko, Iwai Shigenori, Yoshida Shonen, Suzuki Motoshi
Abstract excerpt
Structural differences between class A and B DNA polymerases suggest that the motif B region, a wall of the catalytic pocket, may have evolved differentially in the two polymerase families. This study examines the function of the motif B residues in Saccharomyces cerevisiae DNA polymerase alpha (pol alpha). Effects of the mutations were determined by biochemical analysis and genetic complementation of a yeast...
Topics
- Amino Acid Sequence
- Base Pairing
- Binding Sites
- Catalysis
- Conserved Sequence
- DNA Polymerase I
- DNA, Fungal
- Deoxyguanine Nucleotides
- Gene Deletion
- Gene Library
- Glycine
- Kinetics
- Lysine
- Molecular Sequence Data