Free energy perturbation techniques applied to subtilisin BPN' stability.

doi:10.1007/978-1-4613-0319-0_13

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Article

Free energy perturbation techniques applied to subtilisin BPN' stability.

Advances in experimental medicine and biology - 1 Jan 1996

Barnett B L, Turner C B

Abstract excerpt

The inability to predict the effect of specific mutations on protein stability has been an area of concern to researchers in the field of protein engineering. Small stabilization free energies (5 to 15 kcal/mol) distinguish the native and the denatured states of a protein, making the rational des...

Topics

Computer Simulation
Crystallography, X-Ray
Enzyme Stability
Mutation
Protein Engineering
Protein Folding
Stochastic Processes
Subtilisins
Thermodynamics
Threonine

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