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Article

Self-excising retroviral vectors encoding the Cre recombinase overcome Cre-mediated cellular toxicity.

Molecular cell - 1 Jul 2001

Silver D P, Livingston D M

Abstract excerpt

The Cre-lox system is often used to manipulate sequences in mammalian genomes. We have observed that continuous expression of the Cre recombinase in cultured cells lacking exogenous lox sites caused decreased growth, cytopathic effects, and chromosomal aberrations. Cre mutants defective in DNA cleavage were not geno- or cytotoxic. A self-excising retroviral vector that incorporates a negative feedback loop to...

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PMID: 11511376
DOI: 10.1016/s1097-2765(01)00295-7

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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