Article
Multiplex detection of hotspot mutations by rolling circle-enabled universal microarrays.
Laboratory investigation; a journal of technical methods and pathology - 1 Aug 2001
Ladner D P, Leamon J H, Hamann S, Tarafa G, Strugnell T, Dillon D, Lizardi P, Costa J
Abstract excerpt
Detection of somatic low abundance mutations in early cancer development requires a discriminatory, specific, and high-throughput methodology. In this study we report specific, discriminatory detection of low abundance mutations through a novel combination of rolling circle amplification (Nat Genet 1998; 19:225-232) and PCR ligation detection reaction on a universal oligonucleotide microarray (J Mol Biol 1999;...
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