Article
Genotyping of multiple single nucleotide polymorphisms with hyperbranched rolling circle amplification and microarray.
Clinica chimica acta; international journal of clinical chemistry - 1 Jan 2009
Li Xiujie, Luo Junfeng, Xiao Pengfeng, Shi Xiaolong, Tang Chao, Lu Zuhong
Abstract excerpt
BACKGROUND: The emerging role of single nucleotide polymorphisms (SNPs) in clinical diagnostics and studies has created a need for simple and high-throughput genotyping methods. Previously, we developed a 3-dimensional polyacrylamide gel-based microarray (3-D microarray) of PCR-product. This method can detect single SNP locus from multiple DNA samples on one chip. METHODS: Hyperbranched rolling circle...
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