Article
Prevalence of recombinational versus mutational events in damaged plasmid DNA containing regions of homology with the chromosome.
Mutation research - 1 Nov 1991
Dolzani L, Lagatolla C, Monti-Bragadin C
Abstract excerpt
Plasmid DNA modified by in vitro treatments was transformed in E. coli bacterial cells. A streptomycin-resistant strain, carrying the peculiar rpsL421 mutation, was used as a recipient for the cloning vector pNO1523, which carries the wild-type (streptomycin-sensitive) rpsL allele. Transformants were streptomycin-sensitive unless a change in plasmid sequence had occurred. The analysis of the MaeI restriction...
Topics
- Chromosomes, Bacterial
- Cisplatin
- DNA Damage
- DNA, Bacterial
- Deoxyribonucleases, Type II Site-Specific
- Drug Resistance, Microbial
- Escherichia coli
- Escherichia coli Proteins
- Ethyl Methanesulfonate
- Mutagenesis