Article
A de novo designed peptide ligase: a mechanistic investigation.
Journal of the American Chemical Society - 7 Mar 2001
Kennan A J, Haridas V, Severin K, Lee D H, Ghadiri M R
Abstract excerpt
A 33-residue de novo designed peptide ligase is reported which catalyzes the template-directed condensation of suitably activated short peptides with catalytic efficiencies in excess of 10(5) ([k(cat)/K(m)]/k(uncat)). The ligase peptide, derived from natural and designed alpha-helical coiled-coil proteins, presents a surface for substrate assembly via formation of a hydrophobic core at the peptide interface....
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