Article
A designed peptide ligase for total synthesis of ribonuclease A with unnatural catalytic residues.
Science (New York, N.Y.) - 14 Oct 1994
Jackson D Y, Burnier J, Quan C, Stanley M, Tom J, Wells J A
Abstract excerpt
An engineered variant of subtilisin BPN', termed subtiligase, which efficiently ligates esterified peptides in aqueous solution, was used for the complete synthesis of ribonuclease (RNase) A that contains unnatural catalytic residues. Fully active RNase A (124 residues long) was produced in milligram quantities by stepwise ligation of six esterified peptide fragments (each 12 to 30 residues long) at yields...
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