Article
A designed peptide ligase for total synthesis of ribonuclease A with unnatural catalytic residues.
Science (New York, N.Y.) - 14 Oct 1994
Jackson D Y, Burnier J, Quan C, Stanley M, Tom J, Wells J A
Abstract excerpt
An engineered variant of subtilisin BPN', termed subtiligase, which efficiently ligates esterified peptides in aqueous solution, was used for the complete synthesis of ribonuclease (RNase) A that contains unnatural catalytic residues. Fully active RNase A (124 residues long) was produced in milli...
Topics
- Amino Acid Sequence
- Binding Sites
- Esterification
- Histidine
- Hydrogen-Ion Concentration
- Molecular Sequence Data
- Mutation
- Nucleotides, Cyclic
- Protein Engineering
- Ribonuclease, Pancreatic
- Subtilisins
- Uridine Monophosphate