Article
Installing hydrolytic activity into a completely de novo protein framework.
Nature chemistry - 1 Sept 2016
Burton Antony J, Thomson Andrew R, Dawson William M, Brady R Leo, Woolfson Derek N
Abstract excerpt
The design of enzyme-like catalysts tests our understanding of sequence-to-structure/function relationships in proteins. Here we install hydrolytic activity predictably into a completely de novo and thermostable α-helical barrel, which comprises seven helices arranged around an accessible channel. We show that the lumen of the barrel accepts 21 mutations to functional polar residues. The resulting variant, which...
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