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In-Tube DNA Methylation Profiling by Fluorescence Melting Curve Analysis

1 Jul 2001

Abstract excerpt

BACKGROUND: Most PCR assays for detection of 5-methylcytosine in genomic DNA entail a two-step procedure, comprising initial PCR amplification and subsequent product analysis in separate operations that usually require manual transfer. These methods generally provide information about methylation of only a few CpG dinucleotides within the target sequence. METHODS: An in-tube methylation assay is described that...

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PMID: 11427447
DOI: 10.1093/clinchem/47.7.1183

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