Article
Screening insertion libraries for mutations in many genes simultaneously using DNA microarrays.
Proceedings of the National Academy of Sciences of the United States of America - 19 Jun 2001
Mahalingam R, Fedoroff N
Abstract excerpt
We describe a method to screen pools of DNA from multiple transposon lines for insertions in many genes simultaneously. We use thermal asymmetric interlaced-PCR, a hemispecific PCR amplification protocol that combines nested, insertion-specific primers with degenerate primers, to amplify DNA flanking the transposons. In reconstruction experiments with previously characterized Arabidopsis lines carrying insertions...
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