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Article

Technical advance: display and isolation of transposon-flanking sequences starting from genomic DNA or RNA.

The Plant journal : for cell and molecular biology - 1 Mar 2000

Yephremov A, Saedler H

Abstract excerpt

Insertion mutagenesis using transposons has become a powerful tool for the isolation of genes involved in any given biochemical or developmental pathway. We describe here ligation-mediated PCR techniques for the isolation of sequences flanking the transposable elements En/Spm, Mu1 and Cin4 in Zea mays and Arabidopsis thaliana. Two versions of this transposon insertion display (TID) method use biotinylated linkers...

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PMID: 10758500
DOI: 10.1046/j.1365-313x.2000.00704.x

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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