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Article

Activation tagging using the maize En-I transposon system for the identification of abiotic stress resistance genes in Arabidopsis.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2013

Harb Amal, Pereira Andy

Abstract excerpt

Activation tagging is a high-throughput method of overexpressing genes by using an enhancer present in insertion sequences that are randomly inserted in the genome to enhance the expression of adjacent genes. Gain-of-function approaches are advantageous to identify the functions of redundant genes that are not identifiable by knockout (KO) mutations, and for identification of phenotypes with small effects, which...

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PMID: 23918430
DOI: 10.1007/978-1-62703-568-2_14

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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