Article
A new resource of locally transposed Dissociation elements for screening gene-knockout lines in silico on the Arabidopsis genome.
Plant physiology - 1 Aug 2002
Ito Takuya, Motohashi Reiko, Kuromori Takashi, Mizukado Saho, Sakurai Tetsuya, Kanahara Hiroko, Seki Motoaki, Shinozaki Kazuo
Abstract excerpt
We transposed Dissociation (Ds) elements from three start loci on chromosome 5 in Arabidopsis (Nossen ecotype) by using a local transposition system. We determined partial genomic sequences flanking the Ds elements and mapped the elements' insertion sites in 1,173 transposed lines by comparison with the published genomic sequence. Most of the lines contained a single copy of the Ds element. One-half of the lines...
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