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Article

DLA-based strategies for cloning insertion mutants: cloning the gl4 locus of maize using Mu transposon tagged alleles.

Genetics - 1 Dec 2009

Liu Sanzhen, Dietrich Charles R, Schnable Patrick S

Abstract excerpt

Digestion-ligation-amplification (DLA), a novel adaptor-mediated PCR-based method that uses a single-stranded oligo as the adaptor, was developed to overcome difficulties of amplifying unknown sequences flanking known DNA sequences in large genomes. DLA specifically overcomes the problems associated with existing methods for amplifying genomic sequences flanking Mu transposons, including high levels of...

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Share to Acyltransferases
Share to DNA Transposable Elements
Share to Mutagenesis, Insertional

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Nucleic Acid Amplification Techniques

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Share to Sequence Homology, Nucleic Acid

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Source

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Identifiers

PMID: 19805815
DOI: 10.1534/genetics.109.108936
PMCID: PMC2787415

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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