Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Gene tagging with engineered Ds elements in maize.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2013

Li Yubin, Segal Gregorio, Wang Qinghua, Dooner Hugo K

Abstract excerpt

We describe here protocols for isolating genes in maize using Dissociation (Ds) transposons marked with a green fluorescent protein (GFP) transgene. The introduced marker enables the phenotypic scoring of the nonautonomous element and the anchoring of unique primers on the element to facilitate the isolation of the adjacent DNA by PCR. Transposons such as Ds transpose preferentially to sites closely linked to the...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to DNA, Plant
Share to DNA Transposable Elements
Share to Genes, Plant

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to Genetic Engineering

Share to Green Fluorescent Proteins

Share to Plant Leaves

Share to Plants, Genetically Modified

Share to Zea mays

Source

Identifiers

PMID: 23918422
DOI: 10.1007/978-1-62703-568-2_6

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence. Graph snapshot: 2026-09-29.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Gene tagging with engineered Ds elements in maize.PMID 23918422Read ↗

State II dissociation element formation following activator excision in maize.Semantic proximity Read ↗

Ds insertion mutagenesis as an efficient tool to produce diverse variations for rice breeding.Semantic proximity Read ↗

Transposon tagging using Activator (Ac) in maize.Semantic proximity Read ↗

Ac-immobilized, a stable source of Activator transposase that mediates sporophytic and gametophytic excision of Dissociation elements in maize.Semantic proximity Read ↗

Novel GUS expression patterns following transposition of an enhancer trap Ds element in Arabidopsis.Semantic proximity Read ↗

A Multifunctional Mutagenesis System for Analysis of Gene Function in Zebrafish.Semantic proximity Read ↗

TEAseq-based identification of 35,696 Dissociation insertional mutations facilitates functional genomic studies in maize.Semantic proximity Read ↗

The activator/dissociation transposable elements comprise a two-component gene regulatory switch that controls endogenous gene expression in maize.Semantic proximity Read ↗

Transposition pattern of the maize element Ds in Arabidopsis thaliana.Semantic proximity Read ↗

Germinal transpositions of the maize element Dissociation from T-DNA loci in tomato.Semantic proximity Read ↗

Mapping Ds insertions in barley using a sequence-based approach.Semantic proximity Read ↗

Using MuDR/Mu transposons in directed tagging strategies.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.