Tagged Transcriptome Display (TTD) in indica rice using Ac transposition.

doi:10.1007/s004380100528

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Article

Tagged Transcriptome Display (TTD) in indica rice using Ac transposition.

Molecular genetics and genomics : MGG - 1 Sept 2001

Kohli A, Xiong J, Greco R, Christou P, Pereira A

Abstract excerpt

We have developed a population of transgenic indica rice lines containing the autonomous Activator transposon (Ac) from maize. A transposon excision assay using as the reporter the green fluorescent protein (GFP) gene driven by the ubiquitin promoter was used to monitor Ac excision in various tissues. Our results, based on Ac excision and re-insertion events in 289 independent rice transformants, provide an...

Topics

Base Sequence
Blotting, Southern
Cloning, Molecular
DNA Methylation
DNA Primers
DNA Transposable Elements
DNA, Complementary
Green Fluorescent Proteins
Luminescent Proteins
Mutagenesis, Site-Directed
Oryza
Phenotype
Polymerase Chain Reaction
Transcription, Genetic
Transformation, Genetic

Source

Open in PubMed

Identifiers

PMID: 11589565
DOI: 10.1007/s004380100528

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