Article
An inducible targeted tagging system for localized saturation mutagenesis in Arabidopsis.
Plant physiology - 1 Jan 2005
Nishal Bindu, Tantikanjana Titima, Sundaresan Venkatesan
Abstract excerpt
We describe a system of inducible insertional mutagenesis based on the Ac-Ds family of transposons for targeted tagging in Arabidopsis (Arabidopsis thaliana). In this system, the Ac and Ds elements are carried within the same T-DNA and a heat shock-inducible transposase fusion is utilized to control the levels of transposase gene expression, generating transpositions that can be subsequently stabilized without...
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