Article
In vitro abzyme evolution to optimize antibody recognition for catalysis.
Nature biotechnology - 1 Jun 2001
Takahashi N, Kakinuma H, Liu L, Nishi Y, Fujii I
Abstract excerpt
Enzymes have evolved their ability to use binding energies for catalysis by increasing the affinity for the transition state of a reaction and decreasing the affinity for the ground state. To evolve abzymes toward higher catalytic activity, we have reconstructed an enzyme-evolutionary process in vitro. Thus, a phage-displayed combinatorial library from a hydrolytic abzyme, 6D9, generated by the conventional in...
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