Article
Evolving catalytic antibodies in a phage-displayed combinatorial library.
Nature biotechnology - 1 May 1998
Fujii I, Fukuyama S, Iwabuchi Y, Tanimura R
Abstract excerpt
In vitro affinity maturation for evolving catalytic antibodies has been demonstrated by generating a diverse repertoire of the appropriate complementarity-determining regions on a phage surface. Phage display is followed by a selection based on binding to an altered antigen that was not used at t...
Topics
- Antibodies, Catalytic
- Bacteriophages
- Binding, Competitive
- Enzyme-Linked Immunosorbent Assay
- Evolution, Molecular
- Gene Library
- Immunoglobulin Fab Fragments
- Immunoglobulin Variable Region
- Models, Molecular
- Molecular Mimicry
- Mutation
- Oligonucleotides
- Plasmids
- Protein Engineering