Article
An additional serine residue at the C terminus of rhodanese destabilizes the enzyme.
Archives of biochemistry and biophysics - 15 Jan 2001
Kramer G, Ramachandiran V, Horowitz P, Hardesty B
Abstract excerpt
The rhodanese coding sequence was extended at its 3' end by three base pairs to generate mutants coding for a serine or arginine residue at the carboxyl terminus of the protein. Wild-type and mutant coding sequences were expressed in a cell-free Escherichia coli system by coupled transcription/translation. Predominantly full-length protein was formed in all cases. The amount of protein synthesized was quantified...
Topics
- Animals
- Arginine
- Cattle
- Cell-Free System
- Chaperonin 10
- Chaperonin 60
- Codon
- Enzyme Stability
- Escherichia coli
- Kinetics
- Leucine
- Mutagenesis, Site-Directed