Article
Truncations at the NH2 terminus of rhodanese destabilize the enzyme and decrease its heterologous expression.
The Journal of biological chemistry - 23 Oct 1998
Trevino R J, Tsalkova T, Kramer G, Hardesty B, Chirgwin J M, Horowitz P M
Abstract excerpt
Rhodanese mutants containing sequential NH2-terminal deletions were constructed to test the distinct contributions of this region of the protein to expression, folding, and stability. The results indicate that the first 11 residues are nonessential for folding to the active conformation, but they are necessary for attaining an active, stable structure when expressed in Escherichia coli. Rhodanese species with up...
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