Article
The flexibility of the non-conservative region at the C terminus of D-hydantoinase from Pseudomonas putida YZ-26 is extremely limited.
Applied biochemistry and biotechnology - 1 Mar 2008
Zhang Xue-Yao, Niu Li-Xi, Shi Ya-Wei, Yuan Jing-Ming
Abstract excerpt
We previously reported that a deletion mutant (P478) with a residue Arg deleted at the C terminus of D-hydantoinase (P479) from Pseudomonas putida YZ-26 was dissociated into the monomer from its dimeric state. Based on the above result, a series of mutants of the enzyme with the C-terminal residues either deleted or substituted were prepared. The size-exclusion chromatography and bioactivity assay show that a...
Topics
- Amidohydrolases
- Enzyme Stability
- Hydrogen-Ion Concentration
- Manganese
- Models, Molecular
- Mutant Proteins
- Mutation
- Protein Structure, Tertiary
- Pseudomonas putida
- Temperature
- Zinc