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Article

The impact of single cysteine residue mutations on the replication terminator protein.

Biochemical and biophysical research communications - 31 Oct 2003

Vivian J P, Hastings A F, Duggin I G, Wake R G, Wilce M C J, Wilce J A

Abstract excerpt

We report the structural and biophysical consequences of cysteine substitutions in the DNA-binding replication terminator protein (RTP) of Bacillus subtilis, that resulted in an optimised RTP mutant suitable for structural studies. The cysteine residue 110 was replaced with alanine, valine or serine. Protein secondary structure and stability (using circular dichroism spectropolarimetry), self-association (using...

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Identifiers

PMID: 14559228
DOI: 10.1016/j.bbrc.2003.09.126

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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