Chinese hamster rhodanese cDNA: activity of the expressed protein is not blocked by a C-terminal extension.

doi:10.1006/prep.1995.1091

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Article

Chinese hamster rhodanese cDNA: activity of the expressed protein is not blocked by a C-terminal extension.

Protein expression and purification - 1 Oct 1995

Trevino R J, Hunt J, Horowitz P M, Chirgwin J M

Abstract excerpt

We describe a cDNA from Chinese hamster ovary cells which encodes a protein 91 and 96% identical to bovine and rat mitochondrial rhodaneses, respectively. Recombinant protein was expressed from the cDNA in Escherichia coli, purified to homogeneity, and found to have kinetic properties nearly indi...

Topics

Animals
CHO Cells
Cloning, Molecular
Cricetinae
DNA, Complementary
Escherichia coli
Kinetics
Liver
Mutation
Recombinant Proteins
Sequence Analysis, DNA
Sequence Homology, Amino Acid
Thiosulfate Sulfurtransferase

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