Article
Point mutation of alanine (31) to valine prohibits the folding of reduced lysozyme by sulfhydryl-disulfide interchange.
Protein engineering - 1 Jan 2000
Imoto T, Yamada H, Yasukochi T, Yamada E, Ito Y, Ueda T, Nagatani H, Miki T, Horiuchi T
Abstract excerpt
In the preceding paper in this issue, we described the overproduction of one mutant chicken lysozyme in Escherichia coli. Since this lysozyme contained two amino acid substitutions (Ala31----Val and Asn106----Ser) in addition to an extra methionine residue at the NH2-terminus, the substituted amino acid residues were converted back to the original ones by means of oligonucleotide-directed site-specific...
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