Characterization of sequences involved in mediating degradation of ornithine decarboxylase in cells and in reticulocyte lysate.

Abstract excerpt

Mouse ornithine decarboxylase is a 461-amino-acid protein that is extremely labile. A set of contiguous in-frame deletions were introduced into its C-terminal hydrophilic region. The resulting mutant proteins were expressed in cos monkey cells using an expression vector based on simian virus 40 (SV40) or by in vitro translation in reticulocyte lysate. The degradation of wild-type and mutant proteins was...

Topics

• Animals
• Cells, Cultured
• Mutation
• Ornithine Decarboxylase
• Protein Conformation
• Reticulocytes
• Transfection