Article
Mutation in the interdomain tether influences the stability and refolding of the enzyme rhodanese.
Biochimica et biophysica acta - 27 Sept 1995
Luo G X, Hua S, Horowitz P M
Abstract excerpt
Rhodanese is a single polypeptide chain of 293 amino acids that is folded into two globular domains of nearly equal size that are connected by a 16 amino acid tether. Two amino acids, Val-Asp (VD), were inserted into the interdomain tether through site-directed mutagenesis to produce the new interdomain sequence, E145PSRPEPAIFKAVDTLNR. The purified mutant protein, when unperturbed, was virtually indistiguishable...
Topics
- Amino Acid Sequence
- Base Sequence
- Binding Sites
- Enzyme Stability
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Mutation
- Protein Conformation
- Protein Folding
- Recombinant Proteins
- Serine Endopeptidases