Article
Improved efficiency of mutation detection by denaturing high-performance liquid chromatography using modified primers and hybridization procedure.
Genetic testing - 1 Jan 2001
Narayanaswami G, Taylor P D
Abstract excerpt
The addition of a 20-base GC-clamp to a DNA fragment enabled mutations to be detected by denaturing high-performance liquid chromatography (DHPLC) in the higher melting domain of the two-domain fragment DYS271. The mutations were undetectable in the absence of the GC-clamp. The heteroduplex yield was greatly decreased by the presence of mutations in the high melting domain, presumably because this region anneals...
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