Article
Rapid denaturing high-performance liquid chromatography (DHPLC) for mutation scanning of the transforming growth factor beta3 gene using a novel proof-reading polymerase.
European journal of immunogenetics : official journal of the British Society for Histocompatibility and Immunogenetics - 1 Oct 2003
Bayat A, Walter J M, Lamb H, Ferguson M W J, Ollier W E R
Abstract excerpt
We have utilized a novel variation on the conventional denaturing high-performance liquid chromatography (DHPLC) technology, which we term rapid DHPLC, combining changes in instrumentation, cartridge technology and analysis conditions to enable significant increases in throughput to be achieved. In addition, the use of a novel proof-reading polymerase for sample amplification with a low misincorporation rate...
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