Article
Temperature and pH studies of short tandem repeat systems using capillary electrophoresis at elevated pH.
Electrophoresis - 1 Jan 2001
Nock T, Dove J, McCord B, Mao D
Abstract excerpt
The DNA secondary structure can affect the migration time and precision of DNA separations in the physical gels used in capillary electrophoresis (CE). To counteract these effects, DNA typing is performed using elevated temperatures (60 degrees C) and high concentrations (7 M) of urea. These conditions affect the precision and lifetime of the analysis. To better understand the effects of these conditions on the...
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