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Short tandem repeat typing by capillary array electrophoresis: comparison of sizing accuracy and precision using different buffer systems.

Genomics - 1 Apr 1997

Vainer M, Enad S, Dolnik V, Xu D, Bashkin J, Marsh M, Tu O, Harris D W, Barker D L, Mansfield E S

Abstract excerpt

Polymorphic microsatellite markers are widely used in gene discovery and mapping, human identification, agricultural genetics, and diagnosis of triplet-repeat expansion disorders. Reliable genotyping of these markers requires polymerase chain reaction (PCR) amplification and very-high-resolution electrophoresis. Capillary array electrophoresis offers extremely fast, high-resolution separation of DNA and more...

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PMID: 9126475
DOI: 10.1006/geno.1997.4608

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