Article
The beta-beta-alpha fold: explorations in sequence space.
Journal of molecular biology - 13 Apr 2001
Sarisky C A, Mayo S L
Abstract excerpt
The computational redesign of the second zinc finger of Zif268 to produce a 28 residue peptide (FSD-1) that assumes a betabetaalpha fold without metal binding was recently reported. In order to explore the tolerance of this metal-free fold towards sequence variability, six additional peptides resulting from the ORBIT computational protein design process were synthesized and characterized. The experimental...
Topics
- Algorithms
- Amino Acid Sequence
- Circular Dichroism
- Computer Simulation
- DNA-Binding Proteins
- Hydrogen Bonding
- Magnetic Resonance Spectroscopy
- Models, Molecular
- Molecular Sequence Data
- Mutation
- Peptides