Article
Assessment of the robustness of a serendipitous zinc binding fold: mutagenesis and protein grafting.
Structure (London, England : 1993) - 1 Feb 2005
Sharpe Belinda K, Liew Chu Kong, Kwan Ann H, Wilce Jackie A, Crossley Merlin, Matthews Jacqueline M, Mackay Joel P
Abstract excerpt
Zinc binding motifs have received much attention in the area of protein design. Here, we have tested the suitability of a recently discovered nonnative zinc binding structure as a protein design scaffold. A series of multiple alanine mutants was created to investigate the minimal requirements for folding, and solution structures of these mutants showed that the original fold was maintained, despite changes in...
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