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Article

Stabilization of hen egg white lysozyme by a cavity-filling mutation.

Protein science : a publication of the Protein Society - 1 Feb 2001

Ohmura T, Ueda T, Ootsuka K, Saito M, Imoto T

Abstract excerpt

Stabilization of a protein using cavity-filling strategy has hardly been successful because of unfavorable van der Waals contacts. We succeeded in stabilizing lysozymes by cavity-filling mutations. The mutations were checked by a simple energy minimization in advance. It was shown clearly that the sum of free energy change caused by the hydrophobicity and the cavity size was correlated very well with protein...

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Identifiers

PMID: 11266617
DOI: 10.1110/ps.37401
PMCID: PMC2373952

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