Article
A novel technique for detecting single nucleotide polymorphisms by analyzing consumed allele-specific primers.
Electrophoresis - 1 Feb 2001
Watanabe G, Umetsu K, Yuasa I, Sato M, Sakabe M, Naito E, Yamanouchi H, Suzuki T
Abstract excerpt
We present a simple and rapid polymerase chain reaction (PCR)-based technique, termed consumed allele-specific primer analysis (CASPA), as a new strategy for single nucleotide polymorphism (SNP) analysis. The method involves the use of labeled allele-specific primers, differing in length, with se...
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