Article
[A simple and rapid modified--new method for SNP typing by fragment length discrepant allele specific PCR].
Fa yi xue za zhi - 1 Feb 2005
Huang Dai-xin, Yang Qing-en, Zhao Gui-sen
Abstract excerpt
OBJECTIVE: To establish a new method for single nucleotide polymorphism (SNP) typing based on allele specific PCR: fragment length discrepant allele specific PCR (FLDAS-PCR), and study the influence on specific extension by introducing a mismatch at the third or fourth 3'-terminal base of allele specific primers. METHODS: For SNP loci rs759117 and rs760887, two allele specific forward primers, with different...
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