Article
Typing single-nucleotide polymorphisms using a gel-based sequencer: a new data analysis tool and suggestions for improved efficiency.
Molecular biotechnology - 1 Nov 2003
Jungerius Bart J, Veenendaal A, Van Oost Bernard A, Te Pas Marinus F W, Groenen Martien A M
Abstract excerpt
Single-nucleotide polymorphisms (SNPs) are increasingly used as genetic markers. Although a high number of SNP-genotyping techniques have been described, most techniques still have low throughput or require major investments. For laboratories that have access to an automated sequencer, a single-base extension (SBE) assay can be implemented using the ABI SNaPshot trade mark kit. Here we present a modified protocol...
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