Article
Variable fragment length allele-specific polymerase chain reaction (VFLASP), a method for simple and reliable genotyping.
Molecular and cellular probes - 1 Jun 2023
Tóth Tamás, Csaba Ákos, Bokor Attila, Ács Nándor
Abstract excerpt
Single-nucleotide polymorphism (SNP) is a substitution of a single nucleotide at a specific position in the genome. Until now, 585 million SNPs have been identified in the human genome, and therefore, a widely applicable method is desirable to detect a specific SNP. Herein we report a simple and reliable genotyping assay, which seems to be suitable for medium and small size laboratories, as well, to easily...
Topics
- Humans
- Genotype
- Alleles
- Polymerase Chain Reaction
- Nucleic Acid Amplification Techniques
- DNA Primers
- Polymorphism, Single Nucleotide
