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Article

Exploring the catalytic center of TaqI endonuclease: rescuing catalytic activity by double mutations and Mn2+.

Biochimica et biophysica acta - 9 Mar 2001

Cao W, Lu J

Abstract excerpt

TaqI is a metal-dependent endonuclease that recognizes T(downward arrow)CGA, with the arrow indicating the cleavage site. Mutations at K158 render the enzyme inactive and mutations at K157 significantly reduce DNA cleavage activity (W. Cao and F. Barany (1998) J. Biol. Chem. 273, 33002-33010). Aspartate, glutamate, and histidine substitutions were made at K158 in the wild-type and K157S mutant TaqI endonuclease...

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Source

Identifiers

PMID: 11257528
DOI: 10.1016/s0167-4838(01)00149-2

Genes

CGA

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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