Identification of the acidic residues in the active site of DNA polymerase III.

doi:10.1006/jmbi.1998.2352

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Article

Identification of the acidic residues in the active site of DNA polymerase III.

Journal of molecular biology - 22 Jan 1999

Pritchard A E, McHenry C S

Abstract excerpt

The mechanism of nucleotide addition by DNA polymerases involves two metal ions that are coordinated in the active site by conserved acidic residues. The three acidic residues that chelate Mg2+ in the active site of Escherichia coli DNA polymerase III have been identified as Asp401, Asp403, and A...

Topics

Amino Acid Sequence
Bacterial Proteins
Binding Sites
DNA Polymerase III
Escherichia coli
Kinetics
Magnesium
Manganese
Molecular Sequence Data
Mutagenesis, Site-Directed
Mutation
Protein Conformation
Sequence Alignment

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