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Article

Identification of the acidic residues in the active site of DNA polymerase III.

Journal of molecular biology - 22 Jan 1999

Pritchard A E, McHenry C S

Abstract excerpt

The mechanism of nucleotide addition by DNA polymerases involves two metal ions that are coordinated in the active site by conserved acidic residues. The three acidic residues that chelate Mg2+ in the active site of Escherichia coli DNA polymerase III have been identified as Asp401, Asp403, and Asp555 by site-directed mutagenesis. Candidates for mutagenesis were initially chosen based on absolute conservation of...

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PMID: 9887268
DOI: 10.1006/jmbi.1998.2352

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