Article
Misfolding of a DNAzyme for ultrahigh sodium selectivity over potassium.
Nucleic acids research - 2 Nov 2018
He Yanping, Chen Da, Huang Po-Jung Jimmy, Zhou Yibo, Ma Lingzi, Xu Kexin, Yang Ronghua, Liu Juewen
Abstract excerpt
Herein, the excellent Na+ selectivity of a few RNA-cleaving DNAzymes was exploited, where Na+ can be around 3000-fold more effective than K+ for promoting catalysis. By using a double mutant based on the Ce13d DNAzyme, and by lowering the temperature, increased 2-aminopurine (2AP) fluorescence was observed with addition of both Na+ and K+. The fluorescence increase was similar for these two metals at below 10 mM,...
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