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Article

DNA cleavage by EcoRV endonuclease: two metal ions in three metal ion binding sites.

Biochemistry - 8 Jun 2004

Horton Nancy C, Perona John J

Abstract excerpt

Four crystal structures of EcoRV endonuclease mutants K92A and K38A provide new insight into the mechanism of DNA bending and the structural basis for metal-dependent phosphodiester bond cleavage. The removal of a key active site positive charge in the uncleaved K92A-DNA-M(2+) substrate complex results in binding of a sodium ion in the position of the amine nitrogen, suggesting a key role for a positive charge at...

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Identifiers

PMID: 15170321
DOI: 10.1021/bi0499056

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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