Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Plasmid DNA Vaccines: Investigation of Integration into Host Cellular DNA following Intramuscular Injection in Mice

1 Jan 2000

Abstract excerpt

The primary safety concern for DNA vaccines is their potential to integrate into the host cell genome. We describe an integration assay based on purification of high-molecular-weight genomic DNA away from free plasmid using gel electrophoresis, such that the genomic DNA can then be assayed for integrated plasmid using a sensitive PCR method. The assay sensitivity was approximately 1 plasmid copy/microg DNA...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Immunotherapy and Immune Responses
Share to Influenza Virus Research Studies
Share to Microbial infections and disease research

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Source

Identifiers

PMID: 11251381
DOI: 10.1159/000053993

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 8 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Plasmid DNA Vaccines: Investigation of Integration into Host Cellular DNA following Intramuscular Injection in MicePMID 11251381Read ↗

Plasmid DNA vaccines: assay for integration into host genomic DNA.Semantic proximity Read ↗

Plasmid DNA Malaria Vaccine: The Potential for Genomic Integration after Intramuscular InjectionSemantic proximity Read ↗

Guidance for Industry: Considerations for Plasmid DNA Vaccines for Infectious Disease IndicationsSemantic proximity Read ↗

Using Plasmids as DNA Vaccines for Infectious DiseasesSemantic proximity Read ↗

Plasmid BiopharmaceuticalsSemantic proximity Read ↗

Ensuring safety of DNA vaccinesSemantic proximity Read ↗

Tissue distribution of a plasmid DNA encoding Hsp65 gene is dependent on the dose administered through intramuscular deliverySemantic proximity Read ↗

CpG Methylation of a Plasmid Vector Results in Extended Transgene Product Expression by Circumventing Induction of Immune ResponsesSemantic proximity Read ↗

Gene vaccination with naked plasmid DNA: mechanism of CTL priming.Semantic proximity Read ↗

Intramuscular plasmid DNA injection can accelerate autoimmune responses.Semantic proximity Read ↗

Stable gene transfer to muscle using non-integrating lentiviral vectors.Semantic proximity Read ↗

Engineered DNA plasmid reduces immunity to dystrophin while improving muscle force in a model of gene therapy of Duchenne dystrophySemantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.