Article
Plasmid DNA vaccines: assay for integration into host genomic DNA.
Developments in biologicals - 1 Jan 2000
Ledwith B J, Manam S, Troilo P J, Barnum A B, Pauley C J, Griffiths T G, Harper L B, Schock H B, Zhang H, Faris J E, Way P A, Beare C M, Bagdon W J, Nichols W W
Abstract excerpt
The primary safety concern for DNA vaccines is their potential to integrate into host cellular DNA. We describe a sensitive and quantitative assay for investigating the tissue distribution and integration of plasmid DNA vaccines. By including gonadal tissues in the analysis, the potential for germline transmission is also assessed. At various time points after injection, total DNA is isolated from a variety of...
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